![]() All samples were initially screened for MSH2 loss by scoring TMA spots however for all cases with MSH2 loss on TMA, confirmatory immunostaining for MSH2, MSH6, MLH1 and PMS2 was also performed on standard histologic tissue sections.įull paper Login or join for free to view the full paper. rabbit monoclonal IgG antibody clone SP67 directed against human CD10. Spots without internal control staining were considered ambiguous and not scored. was performed using a Ventana Benchmark Ultra automated immunostainer (Roche). Techniques Used: Immunohistochemistry, Staining. ![]() VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody Package Insert.1015765EN. B, Graphical illustration of the impact of fixation time and fixative on staining intensity. VENTANA anti-PMS2 (A16-4) Mouse Monoclonal Primary Antibody Package Insert.1015766EN Rev D. A spot was considered to show MMR protein loss if any tumor cells in any tumor spot showed MMR protein loss, with intact staining in admixed benign prostate glands and/or surrounding stromal cells, endothelial cells or lymphocytes. A, Representative images (×20 magnification) of Calu-3 lung cancer xenograft tumor tissue stained for c-MET (SP44) following the indicated times of fixation in indicated fixatives. Each tissue microarray spot or standard histologic section containing tumor cells was visually dichotomously scored for presence or absence of cytoplasmic MMR protein signal by a urologic pathologist blinded to the sequencing/MSI testing data (TLL). All samples were incubated with primary antibody after antigen retrieval in CC1 buffer, and primary antibody incubation was followed by detection with the UltraView HRP system (Roche/Ventana Medical Systems, Tucson, AZ). MSH2 IHC used a mouse monoclonal antibody (clone G219-1129), MSH6 IHC used a mouse monoclonal antibody (clone 44), MLH1 IHC used a mouse monoclonal antibody (clone M1) and PMS2 IHC used a rabbit monoclonal antibody (clone EPR3947). In the United States, Japan and Canada, MabThera is marketed as Rituxan.MMR protein IHC was performed on the Ventana Benchmark autostaining system utilizing primary antibodies from Ventana (Roche/Ventana Medical Systems, Tucson, AZ). In Europe, MabThera has recently been approved in paediatric indications in GPA/MPA patients for induction of remission and previously untreated B-NHL, in combination with chemotherapy. MabThera is also approved for the treatment of adults in auto-immune diseases: severe active rheumatoid arthritis, pemphigus vulgaris and severe active granulomatosis with polyangiitis and microscopic polyangiitis (GPA/MPA). Thomas Grogan, the company was acquired by Roche in 2008. In the United States, Europe and in other countries, MabThera is approved for the treatment of adults with the following blood cancers: previously untreated and relapsed/refractory follicular lymphoma, previously untreated diffuse large B-cell lymphoma, and previously untreated and relapsed/refractory chronic lymphocytic leukaemia. Founded as Ventana Medical Systems, Inc., in 1985 by University of Arizona pathologist Dr. MabThera attacks these cells both directly and together with the body’s immune system. Bioz Stars score: 99/100, based on 3 PubMed citations. These cells are central to many blood cancers, including common forms of lymphoma and leukaemia. Rochemouse monoclonal antibody against p16 Mouse Monoclonal Antibody Against P16, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. MabThera was the first therapeutic monoclonal antibody to target cells that have the CD20 marker on their surface. 124 antibody diluent buffer ventana medical systems124 Antibody Diluent Buffer Ventana Medical Systems, supplied by Roche, used in various techniques. Solutions Pharma Products See all Pharma Products The VENTANA PTEN (SP218) Rabbit Monoclonal Primary Antibody is directed against the protein tyrosine phosphatase encoded by the tumor suppressor gene phosphatase and tensin homolog (PTEN) at chromosome 10q23.3, which is also known as the gene mutated in multiple advanced cancers (MMAC1). CONFIRM anti-PR (1E2) Primary Antibody is intended for laboratory use for the qualitative detection of progesterone receptor (PR) antigen in sections of formalin-fixed, paraffin-embedded tissue on a Ventana automated slide stainer with Ventana detection kits and ancillary reagents.
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